Centre for Advanced Histology and Microscopy
The Centre for Advanced Histology & Microscopy (CAHM) empowers researchers with cutting-edge tools and expertise in bioimage analysis, histology, and microscopy. Our state-of-the-art facility allows for the preparation, visualisation, and quantification of biological samples at the cellular and subcellular levels. Researchers can choose to utilise our instruments independently or collaborate with our skilled staff through our fee-for-service options.
Our platforms and research capabilities
CAHM houses 4 main research platforms:
Optical microscopy
Our Advanced Optical Microscopy Platform offers a comprehensive suite of imaging modalities, including wide-field, laser scanning confocal, spinning disk confocal, multi-photon intravital, and super resolution microscopy.
We provide expert services and support for researchers including:
- Tailored guidance: Assistance with experimental design and selecting the ideal microscope for your needs.
- Comprehensive training: In-depth training and ongoing support for all microscopes.
- Versatile imaging: Fee-for-service imaging for fixed or living cells and tissues.
Key Equipment
Confocal Microscopes
- Nikon SoRa Spinning Disk Confocal (with super resolution capability). Equipped with Nikon Ti-E, Yokogawa CSU-W1 spinning disk (50 um and SoRa disks), environmental chamber (Oko Labs) and 2x Andor Sona sCMOS cameras. Laser lines: 405, 445, 488, 515, 561 and 640nm.
- Olympus FV3000 confocal (with live cell imaging capability). Equipped with an Olympus IX83 SSU motorised stage and Ludin environmental chamber. Laser lines: 405, 445, 488, 515, 561, 594, 633nm.
- Olympus FV3000 confocal. Equipped with an Olympus IX83 SSU motorised stage. Laser lines: 405, 488, 561 and 633nm.
- Zeiss Elyra 780 confocal equipped with a Zeiss Observer Z1 microscope and Tokai hit environmental chamber for live cell imaging. Laser lines for confocal: 405, 458, 488, 514 and 633nm
- Nikon C2 confocal equipped with Eclipse Ni-E microscope. Laser lines: 405, 488, 561 and 640nm
Super Resolution Microscope
- Zeiss Elyra PS1 (SIM/3D PALM/dSTORM/TIRF). Equipped with a Zeiss Observer Z1 microscope and Tokai Hit environmental chamber for live cell imaging. Laser lines for TIRF/Super-Resolution: 405, 488, 561 and 633nm.
Multiphoton Microscope
- Olympus FVMPE-RS Upright multiphoton microscope with dual pulsed lasers (Mai Tai and Insight lasers) and a SIM scanner. Equipped with a BX63 microscope, motorised stage, temperature controller, heat pad, and anaesthesia apparatus for intravital imaging.
Widefield Microscopes (all equipped with DIC and epifluorescence)
- Olympus IX83 optimised for ultra-fast image capture. Equipped with motorised Prior stage and Ludin environmental chamber for live cell imaging. Filter wheels enable different combinations of excitation/emission filters.
- Olympus BX53 microscope – upright system for imaging fixed slides.
- Zeiss Axiovert microscope – inverted system for imaging plates/flasks/round dishes.
Automated slide scanner
- Olympus VS120 automated slide scanner equipped with a BX61VS microscope. Brightfield imaging is available. 100 slides capacity.
- SLIDEVIEW VS200 automated slide scanner. Brightfield, polarised and epi-fluorescence imaging (with near IR filter) imaging capabilities. 210 slides capacity.
Key Research Applications
Dynamic Cellular Processes: Real-time analysis of cell-cell interactions, cell death, proliferation, differentiation, and intracellular signalling pathways using time-lapse imaging.
- Cell Fate and Division: Investigation of asymmetric cell division and cell fate determination in real-time across generations with minimal phototoxicity using spinning disk confocal microscopy.
- Subcellular Protein Localization: Super-resolution microscopy for visualizing protein distribution beyond the limitations of conventional confocal microscopy.
- In Vivo Cancer Biology: Examination of cancer metastasis, immune-cancer cell interactions, tumour development, and chemotherapeutic efficacy in vivo using multiphoton microscopy.
- Targeted Photomanipulation: Region-specific photobleaching or photoconversion of fluorescent proteins to study protein kinetics with the SIM scanner on the multiphoton microscope.
Multiplex Immunohistochemistry and Spatial Multi-omics
Multiplex immunohistochemistry and spatial multi-omics are powerful tools for researchers investigating the complex interactions within tissues and cells. These techniques enable a deeper understanding of cellular relationships and biological processes.
Multiplex Immunohistochemistry
By visualising up to eight biomarkers simultaneously on a single tissue section intricate spatial relationships are revealed. We offer a variety of pre-designed human and mouse OPAL panels, including T cell, Pan Immune, and tissue-resident memory T-cell (TRM) panels, as well as custom panel optimization services to meet specific research needs.
Spatial Multi-omics
Our spatial multi-omics platforms enable the analysis of whole transcriptomic and/or proteomic markers while preserving their spatial context. This approach provides valuable insights into complex biological processes and disease mechanisms.
Available Services
- Experimental design consultation.
- Training and support on all instruments.
- Antibody and panel optimisation.
- Fee-for-service staining and scanning.
Key Equipment
- Vectra microscope for spectral separation of multiplex-IHC samples such as OPAL (7-plex).
- Phenoimager HT (formerly known as POLARIS) microscope for spectral separation of multiplex-IHC samples such as OPAL (9-plex).
- Nanostring GeoMX Digital Spatial Profiler for the quantification of up to 570 protein panel or up to whole transcriptomics targets – in collaboration with the Molecular Genomics Core.
- CytAssist – 10X Visium sample preparation – in collaboration with the Molecular Genomics Core.
Image Analysis
Our image analysis service is designed to help researchers extract valuable insights from their microscopy data. We offer a range of tools and resources to support visualisation and quantification of 2D, 3D, and 4D data.
We strive to provide practical solutions that are accessible and easy to use, making image analysis less daunting for researchers who may not have extensive experience in this area.
What We Offer
- Software Access: Our workstations are equipped with a variety of commercial and open-source software packages to suit different analysis needs.
- Guidance and Support: We can help you design analysis pipelines and troubleshoot issues.
- Focused workshops: Empowering researchers and clinicians to master essential analysis skills and gain independence in their projects.
- Fee-for-Service Analysis: If you prefer, we can perform the analysis for you.
Available analysis software
- HALO (IndicaLabs)– commercial whole slide scan 2D image analysis platform with multichannel (chromogenic and fluorescence images) and spatial analysis capabilities, including TMA and FISH image analysis.
- MetaMorph, Imaris, Volocity and Arivis – commercial 2D, 3D and 4D image analysis platforms.
- FIJI/ImageJ and QuPATH – open software with macro writing service available.
- CellSens AI – Olympus image analysis platform with AI capability.
- NIS Elements AI – Nikon image analysis platform with AI capability.
- Zen – Zeiss image analysis platform.
- inFORM and PhenoChart – Vectra 3 and POLARIS image processing platform.
Histology
Advanced Histology Platform
Our Advanced Histology Platform is dedicated to supporting researchers and clinicians by providing comprehensive histology services for paraffin-embedded (FFPE) and cryo-embedded (FF) samples. We offer a complete workflow, from tissue processing and embedding to sectioning and staining, to ensure high-quality results for your research.
Our Services
- Tissue Processing and Embedding: Expert preparation of your samples for sectioning.
- Paraffin and Frozen Sectioning: Precise sectioning of tissues for optimal analysis.
- Training and Access to sectioning microtome and staining facilities for self-use.
- Experimental design assistance and support.
- Coverslipping: Professional finishing of your slides for long-term preservation.
- Routine Histology Staining: Standard staining techniques like Hematoxylin and Eosin (H&E) for general tissue morphology.
- Special Histology Staining: A wide array of stains to visualize specific cellular components and structures, including:
- Massons trichrome, and Picro Sirius red for connective tissue.
- Periodic Acid Schiffs stain for glycogen.
- Carmine Alum stain on whole mounts.
Fee-for-Service Model
Our services are available on a fee-for-service basis, offering flexibility and convenience for your research projects.
Key Equipment
- Tissue Tek VIP 6 and Leica CM3050 S Tissue processors
- Thermo fisher Cryostar NX70 Cryostat
- Sakura Tissue-Tek TEC embedding system
- Leica XL Auto-stainer
- Instrumel GCS600 Coverslipper
- Dako USS Immunostainer
- Epredia HM355S Rotary Microtomes
- Epredia HM340E Rotary Microtome
- Leica RM2255 Rotary Microtome
- Leica Vibratome
- Sysmex cell analyser
Experimental design
At CAHM, we are dedicated to supporting your research journey from start to finish. We offer comprehensive assistance, including grant writing, tailored panel/antibody selection, expert microscope recommendations, and detailed budget estimates. For a personalized consultation, reach out to us at This email address is being protected from spambots. You need JavaScript enabled to view it.. Let's collaborate to achieve your research goals.
Online Resources
We offer a wealth of online resources for the research community, including engaging workshops, powerful analysis software, and convenient online learning modules. Catch up on past sessions through our YouTube channel.
Team Members
- Dr Nicholas Anthony - Manager
- Dr Metta Jana - Deputy Manager & Optical Microscopist
- Dr Rejhan Idrizi - Research Imunohistochemist
- Dr Han Aw Yeang - BioImage Analyst
- Dhanya Menon - Research Histology Team Leader
- Barbara Przybylowski – Research Histologist
- Judy Borg – Research Immunohistochemist
- Leah Adolph – Research Histologist
Contact
For general, optical microscopy, and image analysis enquiries, please contact CAHM Manager, Dr Nicholas Anthony (This email address is being protected from spambots. You need JavaScript enabled to view it.)
For multiplex immunohistochemistry and spatial multi-omics enquiries, please contact CAHM Deputy Manager, Dr Metta Jana (This email address is being protected from spambots. You need JavaScript enabled to view it.)
For Histology enquiries, please contact Research Histology Team Leader, Dhanya Menon (This email address is being protected from spambots. You need JavaScript enabled to view it.)
For all other enquiries, please email This email address is being protected from spambots. You need JavaScript enabled to view it.
Acknowledgements
