The Genotyping Core provides a comprehensive mouse genotyping service, from extraction of genomic DNA from tissue to reporting of genotypes. We also offer Mycoplasma testing of cell lines. We are experts in genotyping and have been providing genotyping services locally for over 10 years. The team is keen to assist researchers by providing a convenient, timely, high-quality and customised service.
Genotyping Core services
Genotyping
- We provide mouse genotyping via a range of methods:
- Conventional PCR
- Real-time PCR, including qPCR and High Resolution Melt analysis (HRM)
- Allele-specific genotyping using Taqman/KASP assays
- Genotyping-by-phenotyping using flow cytometry
- We offer assay development and optimisation based on industry trends and best practice, and can establish new and custom projects, offering expertise in primer design and assay design
Mycoplasma testing
- A qPCR-based test for the rapid and sensitive detection of mycoplasma contamination of cell lines
- Based on established methods using universal primers, we can detect more than 25 different mycoplasma species, including those commonly found in cell culture
Why use us?
The Genotyping Core offers:
- Affordable and competitive pricing
- Fast turn-around time – results within 5-7 working days for routine genotyping
- Convenient and accessible
- Internal users / VCCC building partners:
- Sample drop-off to the Genotyping Core laboratory
- Mycoplasma testing requests and data delivery via iLab
- Mouse genotype results uploaded to Tick@Lab or iLab as required
- External users:
- Sample drop-off at the VCCC, or mail via Express Post
- Mycoplasma testing and mouse genotyping requests and data delivery via iLab
- Internal users / VCCC building partners:
Genotyping Core use
Genotyping and cell line mycoplasma test services are available to all VCCC members and external users. For further information about pricing and sample submission please contact us.
Genotyping Core contact
Jade Cooke
- Phone: (03) 8559 8091
- Email:
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